Abstract: To obtain high precision wind profile and turbulence products and fully understand the application potential of wind lidar, quality control of Doppler wind lidar with five-beam swing (DBS5) mode is investigated in this work using radial velocity and signal-to-noise ratio (SNR). Three-dimensional wind and turbulence measurements by the DBS5 mode of wind lidar are systematically evaluated based on measurements of a three-dimensional ultrasonic anemometer mounted on a tower. The results show that the wind lidar exhibits excellent observational accuracy with root mean square errors (RMSEs) as low as 0.4 m/s, 0.1 m/s, 0.1 m/s, 0.1 m/s, and 0.5 m²/s² for horizontal wind speed (WS), vertical velocity (w), standard deviation of vertical velocity ( \sigma _w ), friction velocity ( u_* ), and turbulent kinetic energy (TKE), respectively at the height of 140 m. Moreover, the impacts of time scale, elevation angle, and spatial scale on observational accuracy of wind lidar are investigated. The results indicate that variations in time scale have little impact on observational accuracy, while elevation angle may affect the accuracy of TKE. Additionally, the RMSEs of WS and TKE gradually increase with increasing spatial scale, while the accuracy of w, \sigma _w , and u_* remain relatively stable. Further investigation of profiles of turbulence and vertical velocity under clear-sky conditions in Beijing indicates that the power spectra of vertical velocity is consistent with the classical − 5/3 scaling law at different periods and heights, whereas white noise appears in the high-frequency region and intensifies with increasing height, especially above the boundary layer. Finally, the vertical turbulence of low-level jets (LLJs) observed by Doppler wind lidar is stronger below the jet height and weaker in and above the jet height.